Abstract : This assessment was grasped to investigate the apportionment of etiological masters and antimicrobial resistance plan among the blood culture-positive cases of neonatal sepsis admitted to a measurement III neonatal thought unit in a demonstrating clinical facility Kolkata, Eastern India for the hour of 4 years (2014 – 2017). Blood culture was cultivated for all newborn children surrendered with clinical sepsis or with positive sepsis screen by means of automated BACTEC 9050 structure. In positive cases, recognizing confirmation of animal was done trailed by antimicrobial affectability testing by Kirby Bauer plate dispersal technique using Clinical Research Center Standard Organization Rules. Multi-quiet impediment (MDR) was described as the closeness of assurance from at any rate three of the five enemy of microbial social occasions if there ought to emerge an event of gram-negative infinitesimal living beings. Chi-squared test was performed to test the connection between different social events. Out of 4781 examples of clinical neonatal sepsis, 415 (8.68%) were culture positive. In 240 (69%) cases, the pathogens had a spot with the family Enterobacteriaceae. Gram-positive cocci speak to 38 (11%) occasions of positive blood culture cases. Klebsiella is the superior powerful administrator with positive in 187(54%) of every single bacterial isolate. Enterobacteriaceae positive youngsters had 90 (36.9%) passing which is in a general sense higher than that related with others. Kl

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