Abstract : Background: several factors are implicated in the occurrence of cancer basing on the degree of DNA damage to which, in turn, is equivalent to the extent of reactive oxygen and nitrogen stresses, 8-Hydroxydeoxyguanosine (8- OHdG) is consider as a marker of oxidative stress in DNA molecule . Objective: This research was conducted to assess serum markers of protein oxidation, DNA damage, and lipid peroxidation levels in ovarian cancer. Patients and methods. The present study is composed of 85 women (mean age = 62.03±12.4 yrs) with clinically and pathologically confirmed ovarian cancer and 65 healthy women as a control group (mean age = 61±12.1 yrs). The measured biochemical parameters included: the level of serum protein oxidation, DNA damage, and lipid peroxidation Results: The results demonstrated significantly high values of 8-OHdG (p<0.0001) and significantly high (p<0.001, p<0.002)values in advanced oxidation protein products (AOPPs) & 3-Nitrotyrosine (3-NT) in ovarian cancer in comparison with control group. There were also significantly high (p<0.05, p<0.001) values of sera, 4-hydroxynonenal 4-(HNE) and (8-isoprostaglandin F2alpha(8-PGF) in women ovarian cancer in comparison with control group. Conclusion: Assessment marker of, DNA damage, lipid peroxidation and protein oxidation May serve as prognostic tools in Ovarian carcinoma and the role of oxidative stress as a carcinogenic factor in ovarian cancer pathogenesis.