Sapporo Medical Journal

Abstract : The characteristics evaluation of the physic (sensory, swelling, disintegration, active ingredient release, bulk density, and mass uniformity), biochemistry, acute toxicity, and accelerated aging of polyphenol/chlorophyll pillulae was presented in the current study. Pillulae contained the polyphenol/chlorophyll-rich concentrated extract of corn by-products in Khanh Hoa province, Vietnam. The biochemistry characteristics were analysed, such as the content of polyphenol, chlorophyll, ascorbic acid, thiamin, tocopherol acetate, alginate content, and Ca2+, bioactivities (total antioxidant activity, reducing power activity, DPPH free radical scavenging activity, catalase activity, formation inhibition of conjugated dienes, low-density lipoprotein-antioxidant activity). Pillulae was tested for acute toxicity and accelerated aging for 30 days at 60oC and checked micro-organism. The results showed that pillulae was non-toxic with a maximum dose of 75 pillulae/kg. The sensory, swelling and active ingredient release of pillulae was good with bulk density (0.6 g/cm3), and mass uniformity (140.12 mg) and polyphenol (31.95 mg), chlorophyll (110.02 µg), ascorbic acid (20.27 mg), thiamin (0.22 mg), tocopherol acetate (1.2 mg), alginate content (20.46 mg), and Ca2+ (12 mg). The storage time of pillulae was estimated to be one year at 25oC through accelerated aging storage for 30 days at 60oC, and only detection of total aerobic bacterial (2.8x102 CFU/g) on the 30th day of accelerated aging.