Abstract : Apple leaves (Ziziphus mauritiana Lam) are a traditional plant, contain numerous bioactive ingredients. The paper focused on polyphenol content and antibacterial activity of acetone, hexane, and ethyl acetate extracts from apple leave Ziziphus mauritiana Lam selecting in Ninh Thuan province, Vietnam on four-strain Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, and Staphylococus aureus. Antibacterial activity of different fractions was evaluated by using minimum inhibitory concentration. The results showed that apple leaves (Ziziphus mauritiana Lam) exhibited high antibacterial activity as the evaluation of antibacterial activity by using MIC method in liquid medium, and MIC of three fractions was in the range of 3.125 to 6.25 mg/ml. Polyphenol content of acetone fraction from apple leaves was 68.96 ± 0.53 mg gallic acid equivalent/g DW. The purity of polyphenols in different fractions was arranged in ascending order, acetone, n-hexane, and ethyl acetate. Apple leaves Ziziphus mauritiana was potential in functional food and pharmaceuticals.