Abstract : Musk is a biological secretion with a unique odor produced by the Moschus moschiferus Linnaeus. Although musk was used as an anti-inflammatory, antibacterial, analgesic, antipyretic, anti-ischemic, and anti-infective in Traditional Mongolian Medicine, it has not been standardized until now. This study aimed to conduct a standardization study with focus on the determination of the quality and safety parameters of musk and evaluate the antipyretic effect of the musk by yeast-induced model of fever. The musk was extracted from the domesticated musk deer from the Musk Deer Breeding Center of the ITMT was used in this study. The content of the muscone in the natural musk was determined by HPLC method. Mice were fevered by injecting 20 mg/kg of Brewer’s yeast subcutaneously. Then, rectal temperature was recorded by thermometer for six (6) hours. In this study, the musk reduced the body temperature significantly after yeast injection compared to the control group (*p<0.01). Suitable conditions of HPLC revealed the presence of muscone in natural musk and the content of muscone in natural musk was determined at 0.31±0.005%. The quality and safety parameters of natural musk were determined: moisture content is at 24.88±0.85%, total ash at 2.62 ± 0.32% while aerobic microbes, yeast, and mold were not detected in the Musk. The standardization indicators of natural musk were defined and the Mongolian National Pharmacopeia Monograph’s draft for natural Musk was developed.