Abstract : In this study, simple and sensitive spectrophotometric method for the determination of Bisoprolol fumarate (BIF) in pharmaceutical formulations was reported. The proposed method was based on the reaction between (BIF) and 1, 2-naphthoquine-4-sulphonate (NQS) at alkaline medium (pH10 ) to form deep red product. Beer’s law was obeyed in the range of (0.4-8) ppm of Bisoprolol tartrate at maximum wavelength of 495 nm. Under optimized reaction conditions, linear regression equation of the calibration curve was y = 0.0789 x + 0.0102 (ppm) with a linear correlation coefficient of 0.9991. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.1365 ppm and 0.4550 ppm, respectively. The method was successfully applied to the determination of (BIF) in pharmaceutical formulations.