Sapporo Medical Journal

Abstract : Spectrophotometric techniques can be used to accurately and rapidly determine the concentration of Nitazoxanide in body fluids (serum and urine) through react with diazonium salt ,which result from hydrolyze amino group of 2-Aminobenzothiazole by hydrochloric acid , to formation a precipitate a yellow-orange azo dye. Showed obeyed beers law between 50- 450 μg.mL-1, Molar absorptivity of 0.4916 x103 L mol-1/cm-1 was measured at 478 nm, . for Sandal's sensitivity 0.0132 has a limit of detection (LOD) of 2.96 μg.mL-1 and a limit of quantification (LOQ) of 9.875 μg.mL-1 . This technique has been successfully used to quantify various concentrations of Nitazoxanide in body fluids .