Sapporo Medical Journal

Abstract : Background: AML is a heterogeneous haematological malignancy , it is the most common acute leukemia in adults. FLT3 is the most common molecular aberration in AML , belongs to the group of class III receptor tyrosine kinases (RTKs) which also include the receptors c-kit and c-fms and PDGFR, mutated in more than one third of the AML cases, subdivided into two major classes :internal tandem duplication(ITD) and tyrosine kinase domain(TKD).FLT3/ITD is associated with higher probability of relapse and worse outcome, and however, there is controversy about its impact on the induction outcome. Objectives: 1.The prevalence of the FLT3/ITD mutation in Iraqi adult de-novo AML patients 2. The effect of the FLT3/ITD mutation on the remission induction outcome (CR rate) 3. The association of this gene with the laboratory tests(WBC, Hb ,PLT ,BM blast %) Patients and methods: This Cohort study comprised 41 adult de-novo AML patients, admitted in Baghdad medical city complex from Jan.2018 to Dec.2019, Each patient was investigated by complete blood count and differential, bone marrow aspiration and biopsy as well as immunophenotyping to confirm the diagnosis of AML.All of them had received the standard (3+7 ) protocol.FLT3 gene was screened by expert genetist using conventional PCR technology with special primers in teaching laboratories/molecular unit in Baghdad medical city complex, just at time of diagnosis . The post-induction assessment was done by examination of bone marrow