Sapporo Medical Journal aims to distribute and expand medical data to the World as well as build a supportive and vibrant community of researchers to connect and explore ideas by publishing articles related to all fields of medicine. Sapporo Medical Journal believes that quality and ethical research. The journal seeks to publish original research articles that are hypothetical and theoretical in its nature and that provide exploratory insights in the following fields but not limited to:
Abstract : Asimple, accurate and controlled spectrophotometricmmethod is studied for the determinationmof hydroxychloroquine (HCQ) both pure and in pharmaceutical preparations. The drug's reaction with the 2,4-dinitrophenylhydrazine (2,4-DNPHz) reagent is the foundation of this mathod. The optimum conditions were studied from the effect of the reagent, oxidized agent and NaOH, the reaction time and the optimum temperature for the formation of the color of azo product and it was in compliance with Beer’s law in the range 2.5 to 22.5 µg/mL at the wavelength 620 nm. the molar absorptivity, Sandellʼs sensitivity, detection limit and quantitation limit were 1048.28L.mol-1 .cm-1, 0.3204μg.cm-2, 0.243 μg.ml-1,, and 0.738 μg.ml-1 respectively. The proposed method has been successfully applied to determination of HCQ in its pharmaceutical preparations. The thermodynamic functions such as enthalpy ∆H, entropy ∆S, and Gibbsmfree energy ∆G were calculated, and it was found that the drug absorption not Spontaneous,mendothermic process and more regular of the system.
Abstract : Diabetes Mellitus is one of the most common syndrome in the world. Many complications result from Diabetes including sexual impairment which highlyaffects quality of life in men. The aim of this work is to formulate and evaluate oro-dispersible tablets (ODTs) contain 500 mg metformin and 5 mg Tadalafil. A successful, simple, valid method of analysis for simultaneous determination of metformin and tadalafil was developed using HPLC with UV detector. Acetonitrile-phosphate buffer pH 3.5 was used as a mobile phase and a successful separation and quantification wer achieved. Linearity, precision, recovery and robustness were all tested according to the ICH guideline of pharmaceutical analysis. Four formulas were prepared, using three types of disintegrants, cross povidone, sodium starch glycolate and cross-linked alginic and tw types of diluents. The powder mix each formula was tested for flowability and compressibility. Then, tablets were compressed by direct compression method and evaluated according to the USP specifications of Oro-dispersible tablets. Results showed that the evaluation of all formulas showed an effect of type of disintegrant and diluent on wetting and disintegration times, where cross povidone gave the shortest disintegration time (52 ±2 seconds) Also, increase concentration of Avicel resulted in increase in disintegration time (from 46±2 to 52±2 seconds). Dissolution test of F4 in pH 1.2 and 6.8 showed very fast dissolution of both drugs were more than 85%
Abstract : Background: Coronavirus Disease 2019 (COVID-19) is a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) related illness that has spread globally and may worsen as pulmonary fibrosis develops. Krebs von den Lungen-6 (KL-6) has been recommended as a marker of COVID-19 and the severity of the illness. It is a chemical that is mostly expressed by injured alveolar type II cells. In order to confirm KL-6 potential as a predictive biomarker of severity in patients with COVID-19, this pilot investigation measured blood concentrations of KL-6. Materials and Methods: One hundred and forty patients with COVID-19 participated in the research. According to the severity of the disease, the COVID-19 patients were classified into three groups: critical disease (n=43), severe disease (n=52), and mild/moderate disease (n=45), with (n=60) healthy volunteers as the control group. Blood samples were taken as well as all of the patients' fundamental clinical and demographic information.
Abstract : Background: Inflammation and immune-mediated reactions are facilitated by the endothelial cell adhesion molecule intercellular adhesion molecule-1 (ICAM-1). In patients with coronavirus disease 2019 (COVID-19), viral infection of endothelial cells brought on by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can result in vascular alterations and boost the expression of ICAM-1, and may be utilized as a biomarker to assess disease severity. The purpose of this study was to determine how serum ICAM-1 levels affected the severity and prognosis of COVID-19. Materials and Methods: 120 patients with COVID-19 participated in the research. According to the severity of the condition, the COVID-19 patients were divided into three groups: critical disease (n = 23), severe disease (n = 37), and mild/moderate disease (n = 60), with 60 healthy volunteers working as the control group. Blood samples were taken as well as all of the patients' fundamental clinical and demographic information.
Abstract : In this study, simple and sensitive spectrophotometric method for the determination of Bisoprolol fumarate (BIF) in pharmaceutical formulations was reported. The proposed method was based on the reaction between (BIF) and 1, 2-naphthoquine-4-sulphonate (NQS) at alkaline medium (pH10 ) to form deep red product. Beer’s law was obeyed in the range of (0.4-8) ppm of Bisoprolol tartrate at maximum wavelength of 495 nm. Under optimized reaction conditions, linear regression equation of the calibration curve was y = 0.0789 x + 0.0102 (ppm) with a linear correlation coefficient of 0.9991. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.1365 ppm and 0.4550 ppm, respectively. The method was successfully applied to the determination of (BIF) in pharmaceutical formulations.