Sapporo Medical Journal

Abstract : Background: Immune thrombocytopenia (ITP) is an autoimmune disorder characterized by heterogeneous bleeding manifestations as well as marked-inter individual variation in response to glucocorticoid (GCs); the 1st line ITP treatment. This may be due to Pgp as steroids outside the intracellular space thus decreasing its intracellular concentration. Methods: The study investigated the relation between single nucleotide protein; the product of ATP binding cassette B1( ABCB1) gene. It is involved in effluxing drugs polymorphism (SNPs) in ABCB1gene and the variable response to oral prednisolone treatment in 100 Egyptian ITP patients. Clinical response was measured during 1st 28 days and compared with C3435T and G2677T SNPs determined by PCR REPLF technique. Results: No significant difference in C3435T between ITP patients and control. Carriers of mutant allele of G2677T were significantly higher in ITP patients compared with the control group (p value :02) suggesting possible role in disease predisposition. Conclusion: There was no significant relation between these two SNPs and age of onset of the disease , initial platelet count, response to steroid therapy .

Abstract : The paper focused on the composition of the phytochemistry, the nutrient, and the mineral, and antioxidant activities of Morinda L. grown in three provinces (the central and the south area) in Vietnam. Phytochemistry (flavonoid, triterpenoid, alkaloid, and tannins), nutrient (protein, fat, carbohydrate, and fiber), minerals (Ca2+, Mg2+, Fe3+, Zn2+, Na+, Cu2+), and antioxidant activities (total, reducing power, and DPPH free radical scavenging) of species Morinda L. grown in Binh Phuoc, Khanh Hoa, and Ca Mau province in Vietnam were evaluated and compared. The results showed that the difference in the nutrient, the minerals, and antioxidant activities between species Morinda L. grown in three provinces occurred (p<0.05). The different species and habitats caused different nutrient, minerals, and antioxidant activities. Different phytochemistry appeared in all Noni samples. Antioxidant activities of species Morinda tomentosa grown in Khanh Hoa province was higher than Morinda citrifolia in Binh Phuoc and Ca Mau province. Minerals content decreased in order Binh Phuoc, Khanh Hoa, and Ca Mau. Nutrient content basing on protein content, Morinda L. grown Ca Mau province was evaluated higher than Binh Phuoc and Khanh Hoa.

Abstract : This study aimed to investigate the optimization of acid gamma-aminobutyric (GABA) content biosynthesis in germinated Mung bean. The effect analysis of soak time, germination temperature, and time on GABA content was using the response surface method (RSM), with a central composite design (CCD) with the support of software JMP version 9.0.2. Results showed that GABA content reaches the highest levels of 3.768± 0.041 mg GABA equivalent/g dry matter as soaking the seeds in tap water. GABA content got the highest value of 4.1006± 0.0326 mg GABA equivalent/g dry matter at the optimization condition (soaking time of 8 hours, germination at 34oC for 26 hours in tap water) and increase of 9.362 times, compared to non-treatment Mung bean seeds. Soaking time, germination time, and germination temperature affected on acid gamma-aminobutyric biosynthesis. GABA is useful for application in the functional food and pharmaceuticals containing GABA.

Abstract : Abstract Context: The prevalence of anxiety and depression in chronic lung diseases is not uncommon.Most recent estimates of depression among COPD patients include a prevalence range of 37% to 71%. Depressed patients with chronic lung disease have a higher mortality rate, repeated exacerbations, more frequent hospitalizations and longer hospital stay as compared with non-depressed patients This study aims to study the frequency of depression and anxiety and to detect their impact on quality of life in patients with chronic lung diseases. Settings and Design: This is a cross sectional observational study on patients recruited from the chest department of chest and cardiothoracic hospital of Minia university, Egypt in the period from Septemper,2017 to Septemper,2018 Methods and Material: All patients were subjected to full history taking,clinical examination , mMRC , chest X ray ,HRCT was done whenever needed), The Hamilton Anxiety Rating Scale (HAMA). Beck Depression Inventory (BDI-II)’’ questionnaire and SGRQ . Statistical analysis: Statistical analysis was performed using SPSS software ( χ2 test and independent sample t-test). Results: The frequency of depression and anxiety in all patient groups was 78.7% and 16.4% respectively. Both depression and anxiety scores were positively correlated with age , dyspnea scale,FVC and HRQL score( P 0.001). Conclusion: Depression is more frequent than anxiety in patients with chronic pulmonary diseases .IPF and bronchiectasis grou

Abstract : Early childhood caries or ECC is the most dominant disease in childhood that affecting many children in each country of the world. This study aimed at the application of response surface methodology (RSM) to model the risk factor contributes to early childhood caries based on the contour plot. Contour plot is a helpful visualization of the surface when the factors are no more than three. When there are more than three design variables, it is almost impossible to visualize the surface. The result shows only three variables have the relationship between the number of caries such as the status of caries (5.0973, p = 0.000), the income of the family (0.0009, p = 0.003) and types of water (-0.7927, p = 0.049). On the other hand, severe ECC experience is an important predictor of caries among children. High-income families can buy more sweets and snacks than others which is a contributory factor in creating dental caries among children. Through this study, the significant result is very important especially toward early children and especially their parents to prevent this problem. On the other hand, the government and health professional organizations should monitor the groundwater quality regularly which is used directly without treatment as drinking water.