Sapporo Medical Journal

Abstract : This study aimed to investigate the optimization of acid gamma-aminobutyric (GABA) content biosynthesis in germinated Mung bean. The effect analysis of soak time, germination temperature, and time on GABA content was using the response surface method (RSM), with a central composite design (CCD) with the support of software JMP version 9.0.2. Results showed that GABA content reaches the highest levels of 3.768± 0.041 mg GABA equivalent/g dry matter as soaking the seeds in tap water. GABA content got the highest value of 4.1006± 0.0326 mg GABA equivalent/g dry matter at the optimization condition (soaking time of 8 hours, germination at 34oC for 26 hours in tap water) and increase of 9.362 times, compared to non-treatment Mung bean seeds. Soaking time, germination time, and germination temperature affected on acid gamma-aminobutyric biosynthesis. GABA is useful for application in the functional food and pharmaceuticals containing GABA.

Abstract : Abstract Context: The prevalence of anxiety and depression in chronic lung diseases is not uncommon.Most recent estimates of depression among COPD patients include a prevalence range of 37% to 71%. Depressed patients with chronic lung disease have a higher mortality rate, repeated exacerbations, more frequent hospitalizations and longer hospital stay as compared with non-depressed patients This study aims to study the frequency of depression and anxiety and to detect their impact on quality of life in patients with chronic lung diseases. Settings and Design: This is a cross sectional observational study on patients recruited from the chest department of chest and cardiothoracic hospital of Minia university, Egypt in the period from Septemper,2017 to Septemper,2018 Methods and Material: All patients were subjected to full history taking,clinical examination , mMRC , chest X ray ,HRCT was done whenever needed), The Hamilton Anxiety Rating Scale (HAMA). Beck Depression Inventory (BDI-II)’’ questionnaire and SGRQ . Statistical analysis: Statistical analysis was performed using SPSS software ( χ2 test and independent sample t-test). Results: The frequency of depression and anxiety in all patient groups was 78.7% and 16.4% respectively. Both depression and anxiety scores were positively correlated with age , dyspnea scale,FVC and HRQL score( P 0.001). Conclusion: Depression is more frequent than anxiety in patients with chronic pulmonary diseases .IPF and bronchiectasis grou

Abstract : Early childhood caries or ECC is the most dominant disease in childhood that affecting many children in each country of the world. This study aimed at the application of response surface methodology (RSM) to model the risk factor contributes to early childhood caries based on the contour plot. Contour plot is a helpful visualization of the surface when the factors are no more than three. When there are more than three design variables, it is almost impossible to visualize the surface. The result shows only three variables have the relationship between the number of caries such as the status of caries (5.0973, p = 0.000), the income of the family (0.0009, p = 0.003) and types of water (-0.7927, p = 0.049). On the other hand, severe ECC experience is an important predictor of caries among children. High-income families can buy more sweets and snacks than others which is a contributory factor in creating dental caries among children. Through this study, the significant result is very important especially toward early children and especially their parents to prevent this problem. On the other hand, the government and health professional organizations should monitor the groundwater quality regularly which is used directly without treatment as drinking water.

Abstract : Abstract—Health monitoring of radiation workers due to radiation exposure can be done by examination of the hematopoietic system, especially white blood cells which are very sensitive to radiation exposure and can be used as an indicator of body damage. The purpose of this study was to determine the differences in the biological effects of ionizing radiation on the hematopoietic system of radiation workers and non-radiation workers as controls. A total of 3 mL of blood from 57 people (34 radiation workers and 23 subjects as a control) were taken using a syringe and then put into an EDTA tube and shaken slowly to avoid clotting. The blood was examined using the ABX Micros 60 hemoanalyzer instrument with standard procedure in the PTKMR Laboratory. An independent sample T-test was used to determine the significant differences between radiation workers and controls. Pearson chi-square test was used to evaluate the significance of each parameter through p-value (p<0.05). The results showed that the numbers of hemoglobin, erythrocytes, leukocytes, platelets, hematocrits, MCV, MCH, MCHC, RDW, MPV, PDW, lymphocyte, monocytes, granulocytes, % lymphocytes, % monocytes, % granulocytes of radiation workers did not decrease and still within normal limit. The results of the statistical test did not show a significant difference between radiation workers and controls (p> 0.05). It was concluded that radiation workers did not have any impact due to work with radiation.

Abstract : Bacterial activity is one of the factors involved in the pathogenesis of acne vulgaris (AV). Previous studies reported that other bacteria found in addition to P. acnes in AV lesions, may also play a role in acne pathogenesis. Furthermore, an increase in antibiotic resistance towards these bacteria become problems. The aim of this study was to identify bacteria and determine antibiotic resistance from comedones of AV patients in Dr. Hasan Sadikin General Hospital West Java, Indonesia. A descriptive cross-sectional study was undertaken from January to February 2019. A total of 30 samples were collected from closed comedone, then cultured under aerobic and anaerobic conditions. Species identification was done by Vitek® 2 compact. The isolates were tested for resistance to nine antibiotics by disk diffusion methods. The result of this study consisted of 53.6% P. acnes, 17.9% Staphylococcus epidermidis (S. epidermidis), 10.7% Staphylococcus hominis ssp. hominis (S. hominis spp. hominis), 7.1% Staphylococcus capitis (S. capitis), 1,8% for each of Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus warneri, Pseudomonas aeruginosa, Klebsiella pneumoniae spp. pneumoniae, and Enterobacter aerogenes. The two most common bacteria in one comedone were P. acnes concurrently with S. epidermidis. The highest antibiotic resistance of all bacteria was against clindamycin (62.5%), azithromycin (60.7%), erythromycin (57.1%), cotrimoxazole (46.4%), tetracycline (28.6%), levofloxac