Sapporo Medical Journal aims to distribute and expand medical data to the World as well as build a supportive and vibrant community of researchers to connect and explore ideas by publishing articles related to all fields of medicine. Sapporo Medical Journal believes that quality and ethical research. The journal seeks to publish original research articles that are hypothetical and theoretical in its nature and that provide exploratory insights in the following fields but not limited to:
Abstract : Preeclampsia is the leading cause of morbidity and mortality worldwide. Etiology, and pathophysiology are poorly understood with a recent theory as two stages disorder. The main hypothesis result from a defect in remodelling of spiralis artery, while late-onset preeclampsia is generally considered as maternal factor preeclampsia. Delivery remain the main treatment option, as ACOG already recommended low dose aspirin for high risk pregnancy to reduce mortality rate. Lipid abnormalities has effect on endothelial function which is the main pathogenesis and risk factor of preeclampsia. In pregnancy, lipid metabolism changes occured for fetal growth and development. Pregnant women with preeclampsia has significantly higher lipid profile. Statin is considered safe and has advantages in treating dyslipidemia. Randomized clinical trial were done to assess effect of pravastatin in 71 high risk preeclampsia pregnant women. Clinical trial were done in two groups. Control group (Group A) take 80mg aspirin only as recommended by ACOG until 36 week of pregnancy. Intervention group (Group B) take aspirin and additional 20mg pravastatin twice daily since 18 weeks until 36 week of pregnancy. Maternal lipid profile before and after treatment on both groups were assesed. Maternal outcome and baby outcome were assessed on both groups. There is no significant difference viewed from between groups on initial test of lipid profile. Even after therapy, total cholesterol and triglyceride keep on incr
Abstract : The incidence of premature rupture of membranes is approximately 12% of all pregnancies, although this varies between 2-18% of pregnancies. The pathogenesis and epigenetic aspects of premature rupture of membranes are still unclear. lncRNA, ADAMTS15 mRNA and collagen are associated with premature rupture of membranes (PROM). Thus, this study would like to analyze the correlation of lncRNA BF328678, ADAMTS15 mRNA expression and collagen levels in premature rupture of membranes. The samples taken from two groups of subject, that consist of 20 people with premature rupture of membranes and 20 people without premature rupture of membrans. The mean of lncRNA expression in spontaneous labor with premature rupture of membranes is: 139.1 + 28.94, compared to spontaneous labor without premature rupture of membranes, is: 267.2 + 55.84, with p < 0.05. The mean of ADAMTS15 mRNA expression in spontaneous labor with premature rupture of membranes is: 39.4 + 10.01, compared with the results of expression on spontaneous labor without premature rupture of membranes, is: 6.95 + 2.32, with p < 0.05. The mean of collagen levels in spontaneous labor samples with premature rupture of membranes is: 5.92 + 1.95, compared to spontaneous labor without PROM, is: 6.95 + 2.32, with p < 0.05. It can be concluded that premature rupture of membranes increased expression of ADAMTS 15 mRNA.
Abstract : Background: Hepatic encephalopathy HE is a neuropsychological disorder in patient with advanced liver disease. The ammonia has a critical role in HE pathogenesis. The aim: to evaluate efficacy and safety of sodium benzoate (SB) as a cheap adjunctive agent that can be used in addition to rifaximin or lactulose for the treatment of HE. Methods: This study included 90 patients of overt HE in 3 groups: Group A (30 patients received oral sodium benzoate capsule and lactulose), group B (30 patients received oral sodium benzoate capsules and rifaximin, and group C (30 patients (control group) received lactulose and rifaximin). Each group was subjected to clinical examination, and laboratory investigations. The three groups evaluated before and after treatment for clinical response using West Haven Criteria classification of HE, Clinical Hepatic Encephalopathy Staging Scale (CHESS) score and psychometric tests. Results: There was statistically significant difference in WHC grades of HE, CHESS score of HE, and psychometric tests before and after treatment in each group (P < 0.05). There was no statistically significant difference in the overall improvement between the three studied groups after the treatment regarding WHC grades, CHESS score and psychometric tests. No reported significant adverse events. Conclusion: Adding SB to either lactulose or rifaximin in the treatment of HE is clinically equivalent to lactulose and rifaximin combination. This finding highlights the therapeutic
Abstract : Aim of the work: to clinically describe patients with COVID-19 who presented to El-Minia university hospital screening triage and to Clarify the simplest way to predict the severity of COVID-19. Patients and methods: the current study is a retrospective study on the clinical criteria and laboratory results including inflammatory and coagulation markera (NLR, LMR, CRP, ferritin and D-dimer) of 76 patients of COVID-19. The patients classified based on the severity into severe and non-severe group. Results: the median age of studied patient was 47 years old of them 43 male and 33 female. The risk of severity increased with hypertension and chronic kidney disease. As regards laboratory results in WBCs, neutrophil percentage and count, NLR, ALT, AST, urea and creatinine Significantly increased also there was an increase in inflammatory markers as CRP, ferritin and D-dimer with decrease in Hb, lymphocytes percentage, monocytes percentage level in severe group comparing to non-severe group. By applying binary logistic regression analysis it was found that increase in WBCs, Neutrophil count, NLR, ALT, Urea, Creatinine, CRP, Ferritin and D-dimer increasing the progression and severity of COVID-19. Conclusion: lymphopenia and NLR especially CRP, ferritin also, inflammatory markers, is an effective and reliable indicator of the severity and indication of hospitalization in COVID-19 patients.
Abstract : Adipose-derived stem cells (ADSCs) are promising stem cell for autologous regenerative therapy. However, culturing process of ADSCs manipulated the size of cells into senescence formed. The aim of this study was to investigate the effect of concentration variation of ascorbic acid towards proliferation of human ADSCs. Human ADSCs were isolated by enzymatic digestion and cultured in presence serum. To evaluate trilineage ability of ADSCs, multipotency assay into adipocyte, chondrocyte and osteocyte were conducted. The prepared cells then cultured into 8 groups involved cells cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) only, DMEM with 10% foetal bovine serum (FBS) (DMFA), DMFA with 25, 50, 75, 100, 125, and 150 µg/ml of ascorbic acid, respectively. Morphology observation including spread size of cells, population doubling time, viability and proliferation curve were performed. Analysis of type 1 and type 2 collagen and vascular endothelial growth factor (VEGF) as well were conducted in mRNA level using quantitative-real time PCR. The results showed that the cells had plastic adherence capacity and could be differentiated into adipocyte, chondrocytes, and osteocyte. After 120 hours of observation, the cells which cultured with FBS without ascorbic acid supplementation has the highest spread cells in size, lowest in cell yield and lowest in doubling time while the lowest spread size cells with the highest of cell yield and fastest in doubling time was in group with 75 µ